منابع مشابه
Total internal reflection fluorescence.
Total internal reflection fluorescence (TIRF) is an optical effect particularly well-suited to the study of molecular and cellular phenomena at liquid/solid interfaces. Such interfaces are central to a wide range of biochemical and biophysical processes: binding to and triggering of cells by hormones, neurotransmitters, and antigens; blood coagulation at foreign surfaces; electron transport in ...
متن کاملTotal internal reflection Raman spectroscopy.
Total internal reflection (TIR) Raman spectroscopy is an experimentally straightforward, surface-sensitive technique for obtaining chemically specific spectroscopic information from a region within approximately 100-200 nm of a surface. While TIR Raman spectroscopy has long been overshadowed by surface-enhanced Raman scattering, with modern instrumentation TIR Raman spectra can be acquired from...
متن کاملAmplified total internal reflection: theory, analysis, and demonstration of existence via FDTD.
The explanation of wave behavior upon total internal reflection from a gainy medium has defied consensus for 40 years. We examine this question using both the finite-difference time-domain (FDTD) method and theoretical analyses. FDTD simulations of a localized wave impinging on a gainy half space are based directly on Maxwell's equations and make no underlying assumptions. They reveal that ampl...
متن کاملTotal internal reflection fluorescence (TIRF) microscopy.
Total internal reflection fluorescence (TIRF) microscopy (TIRFM) is an elegant optical technique that provides for the excitation of fluorophores in an extremely thin axial region ("optical section"). The method is based on the principle that when excitation light is totally internally reflected in a transparent solid (e.g., coverglass) at its interface with liquid, an electromagnetic field, ca...
متن کاملTotal internal reflection: a deeper look.
In the present paper, we have presented a Maxwellian boundary-type solution for total internal reflection with unbounded incident waves at an interface between two nonabsorbing media, in which the instantaneous, time varying, and time averaged radiant fluxes have been determined at all points in the two media. Solutions for the s and p polarizations were found for which the instantaneous tangen...
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ژورنال
عنوان ژورنال: Optics Express
سال: 2003
ISSN: 1094-4087
DOI: 10.1364/oe.11.000299